By Laurent Roth, Charles Tesson, Jean-Michel Frodon, Alain Bergala
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Extra resources for Abbas Kiarostami : Textes, entretiens, filmographie complete
The higher salt concentration showed a twofold increase in resolution and a fivefold increase in efficiency. The efficiency declines at moderate ionic strength, which causes a lack of resolution for basic proteins with the complete absorption of lysozyme at neutral pH (Swedberg, 1990). Although it has been shown that flow rate varies inversely with buffer concentration (Altria and Simpson, 1986), this was not the case in the example above. A longer migration time obtained using a lower salt concentration buffer is primarily due to adsorption of these positively charged proteins to the capillary wall, which slows the electroosmotic flow.
Summary Capillary electrophoresis is a simple technique with many possible analysis modes. In this book, we will focus on methods of separation and detection that are relevant to the areas of pharmaceutical and biomedical analysis. The field of CE has grown considerably over the past 15 years, and it is anticipated that this growth will persist in the future. In this volume, we have attempted to emphasize fundamentals that will continue to be useful to workers as the field changes and advances.
This is possible only in capillaries with no charge or constant charge at the surface. To this end, modification of capillaries by either masking or deactivating surface silanol groups has been performed by physically coating capillary walls. The effects shown in Fig. 6 are a good example of the reduction and leveling of the EOF over a wide pH range using a coated capillary (Towns and Regnier, 1992a). The pH independence of EOF was also observed with a negatively charged coating (sulfonic acid groups) (Kohr and Engelhardt, 1991).
Abbas Kiarostami : Textes, entretiens, filmographie complete by Laurent Roth, Charles Tesson, Jean-Michel Frodon, Alain Bergala